管徒晨;滕龙;郭贝贝;刘炎;

• 1:南通大学教育部/江苏省神经再生重点实验室/神经再生协同创新中心

摘要(Abstract)：

目的:探究二甲双胍(metformin, MET)对原代皮层神经元轴突生长的效应,以寻找安全有效的促进神经元再生的药物,用于恢复中枢神经系统损伤。方法:原代培养大鼠胚胎18 d(E18)皮层神经元,加入不同浓度MET处理,采用免疫荧光观察神经元轴突生长状况,细胞计数试剂盒8(cell counting kit-8, CCK-8)检测细胞活力变化,利用硫酸软骨素蛋白多糖(chondroitin sulfate proteoglycans, CSPGs)构建神经元轴突生长抑制模型,免疫荧光观察MET是否可以缓解CSPGs的抑制作用。结果 :100μmol/L浓度的MET能够有效促进原代皮层神经元轴突的生长和再生;相比于对照组,MET作用48 h能显著提升细胞活力;MET能够缓解CSPGs对神经元轴突生长的抑制作用。结论:MET可有效促进原代皮层神经元轴突生长及损伤轴突的再生,且能有效缓解CSPGs对皮层神经元生长的抑制作用。

关键词(KeyWords)： 皮层神经元;二甲双胍;轴突生长;硫酸软骨素蛋白多糖;大鼠

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(31970412);; 江苏省自然科学基金面上项目(BK20191446)

作者(Authors): 管徒晨;滕龙;郭贝贝;刘炎;

DOI: 10.16424/j.cnki.cn32-1807/r.2021.03.001

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