血清浓度对皮肤源性前体细胞在生物材料上增殖生长的影响Effect of media with different serum concentration on proliferation and growth of skin-derived precursors cultured on biomaterials
程晓;王澍;谷苗;王钰;何江虹;
摘要(Abstract)：
目的:探讨血清浓度对皮肤源性前体细胞(skin-derived precursors, SKPs)在蝶翅及壳聚糖膜等生物材料表面增殖生长的影响。方法:采用酶消化法从新生大鼠背部皮肤分离纯化SKPs,将传代后的SKPs分别与具有不同表面结构的蝶翅和壳聚糖膜材料共培养,各组材料分别采用含有1%、5%、10%胎牛血清(fetal bovine serum, FBS)浓度的培养基条件,培养4、24、48 h后以细胞活性检测试剂盒(cell counting kit-8, CCK-8)检测各组材料上细胞活力,培养48 h采用倒置显微镜观察SKPs在材料上的贴壁和生长情况。结果:分离纯化的SKPs在各组材料上均能贴壁生长。在蝶翅材料上培养4、24 h后,CCK-8检测结果显示在含有1%FBS浓度培养基条件下SKPs的活力显著低于5%和10%FBS,培养48 h后倒置显微镜下观察,可见1%FBS条件下,细胞大量裂解出现碎片,5%和10%FBS培养条件下,SKPs均生长形态正常,两种蝶翅材料间差异无统计学意义。与蝶翅材料相似,SKPs在具有不同表面形貌的壳聚糖膜各组材料上培养,在1%FBS条件下各种表面形貌壳聚糖膜上SKPs的活力均显著低于5%和10%FBS,且培养48 h,细胞出现大量裂解碎片,5%和10%FBS下SKPs生长形态正常。结论:在具有表面微纳拓扑结构的生物材料上,1%低血清浓度不利于SKPs的生长,5%较低血清浓度可以满足SKPs细胞增殖生长的需要。
关键词(KeyWords)： 血清;皮肤源性前体细胞;蝶翅;壳聚糖膜;细胞增殖
基金项目(Foundation): 国家自然科学基金资助项目(31971276);; 江苏省高校自然科学基金重点资助项目(19KJA320005)
作者(Authors): 程晓;王澍;谷苗;王钰;何江虹;
DOI: 10.16424/j.cnki.cn32-1807/r.2021.05.001
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