李石营;陈褚;刘倩倩;王星辉;冯倩;丁斐;顾晓松;

• 1:南通大学江苏省神经再生重点实验室

摘要(Abstract)：

目的:探讨坐骨神经横断后,microRNA-34a(miR-34a)对c-Met(肝细胞生长因子受体)的调控作用及对施万细胞增殖的影响。方法:采用Real-time PCR检测miR-34a和c-Met在坐骨神经横断后近侧端的表达变化;Edu染色法检测miR-34a对原代培养的施万细胞增殖能力的影响;双荧光素酶报告基因系统检测miR-34a对c-Met 3’UTR的直接靶向作用。结果:miR-34a与c-Met在坐骨神经横断后近侧端的表达呈负相关,能够抑制原代培养的施万细胞的增殖,并可直接作用于c-Met基因的3’UTR。结论:miR-34a可以直接靶向c-Met的3’UTR,从而通过下调靶基因c-Met的表达抑制施万细胞的增殖。

关键词(KeyWords)： microRNA-34a;c-Met;施万细胞;增殖

Abstract:

Keywords:

基金项目(Foundation): 国家863计划项目(2012AA020502);; 国家自然科学基金资助重点项目(81130080);; 江苏省自然科学基金资助项目(BK2012230);; 江苏省普通高校研究生科研创新计划项目(CXZZ12_0862)

作者(Authors): 李石营;陈褚;刘倩倩;王星辉;冯倩;丁斐;顾晓松;

参考文献(References)：

• [1]Li S,Yu B,Wang S,et al.Identification and functionalanalysis of novel micro-RNAs in rat dorsal root ganglia aftersciatic nerve resection[J].J Neurosci Res,2012,90(4):791-801.
• [2]Li S,Yu B,Wang Y,et al.Identification and functionalannotation of novel microRNAs in the proximal sciaticnerve after sciatic nerve transaction[J].Sci China Life Sci,2011,54(9):806-812.
• [3]Zhou S,Yu B,Qian T,et al.Early changes of microRNAsexpression in the dorsal root ganglia following rat sciaticnerve transaction[J].Neurosci Lett,2011,494(2):89-93.
• [4]Yu B,Zhou S,Wang Y,et al.Profile of MicroRNAs follow-ing rat sciatic nerve injury by deep sequencing:implicationfor mechanisms of nerve regeneration[J].PLoS ONE,2011,6(9):e24612.
• [5]Yu B,Zhou S,Qian T,et al.Altered microRNA expressionfollowing sciatic nerve resection in dorsal root ganglia ofrats[J].Acta Biochim Biophys Sin(Shanghai),2011,43(11):909-915.
• [6]Yu B,Zhou S,Wang Y,et al.miR-221/222 promote Schwanncell proliferation and migration by targeting LASS2 follow-ing sciatic nerve injury[J].J Cell Sci,2012,125(Pt11):2675-2683.
• [7]Li N,Fu H,Tie Y,et al.miR-34a inhibits migration and invasion by down-regulation of c-Met expression in human hepatocellular carcinoma cells[J].Cancer Lett,2009,275(1):44-53.
• [8]Chen C,Ridzon DA,Broomer AJ,et al.Real-time quan-tification of microRNAs by stem-loop RT-PCR[J].Nucleic Acids Res,2005,33(20):e179.
• [9]Kim J,Krichevsky A,Grad Y,et al.Identification of many microRNAs that copurify with polyribosomes in mammalian neurons[J].Proc Natl Acad Sci U S A,2004,101(1):360-365.
• [10]Natera-Naranjo O,Aschrafi A,Gioio AE,et al.Identifica-tion and quantitative analyses of microRNAs located in the distal axons of sympathetic neurons[J].RNA,2010,16(8):1516-1529.
• [11]Fiore R,Schratt G.MicroRNAs in vertebrate synapse de-velopment[J].Scientific World Journal,2007,7:167-177.
• [12]Schratt G.microRNAs at the synapse[J].Nat Rev Neurosci,2009,10(12):842-849.
• [13]Cooper CS,Blair DG,Oskarsson MK,et al.Characteriza-tion of human transforming genes from chemically trans-formed,teratocarcinoma,and pancreatic carcinoma cell lines[J].Cancer Res,1984,44(1):1-10.