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目的：探索骨形态发生蛋白(bone morphogenetic protein, BMP)信号通过成纤维细胞生长因子20(fibroblast growth factor 20, FGF20)对多疣壁虎断尾再生的作用机制。方法：构建多疣壁虎断尾再生模型，采用免疫荧光组织化学方法检测BMP信号在12 d再生组织中的表达情况。断尾后隔天腹腔注射BMP信号抑制剂，在第12天观察断尾再生情况，并采用免疫荧光组织化学方法检测FGF20的表达。在体外培养芽基细胞中加入BMP激动剂，采用免疫荧光细胞化学方法检测FGF20表达变化。通过双荧光素酶报告基因和染色质免疫沉淀实验检测SMAD1/5/9对FGF20的转录调控作用。结果：断尾后12 d,BMP4/7和BMPR1A在愈伤上皮和芽基细胞中均有表达，阻碍BMP信号显著抑制尾巴再生以及FGF20的表达。体外培养芽基细胞中激活BMP信号可上调FGF20的表达。SMAD1对FGF20起到正转录调控作用。结论：BMP信号通过SMAD1正转录调控FGF20的表达，进而参与调控多疣壁虎的尾巴再生。
Abstract：Objective: To explore the mechanism by which bone morphogenetic protein(BMP) signaling regulates fibroblast growth factor 20(FGF20) during tail regeneration in Gekko japonicus. Methods: A tail regeneration model was established in Gekko japonicus. Immunofluorescence histochemistry was used to detect the expression of BMP signaling in the regenerating tissue on days 12. After tail amputation, BMP signaling inhibitors were injected intraperitoneally every other day and the regenerating was then evaluated on days 12. The expression of FGF20 was then detected by immunofluorescence histochemistry. In vitro cultured blastemal cells were treated with BMP agonists and immunofluorescence cytochemistry was used to detect FGF20 expression. Dual luciferase reporter system and chromatin immunoprecipitation assay were used to analyze the transcriptional regulation of FGF20 by SMAD1/5/9. Results: BMP4/7 and BMPR1A were expressed in the regeneration tissue on days 12 after tail amputation. Inhibition of BMP signaling significantly inhibited tail regeneration and the expression of FGF20. In vitro, activation of BMP signaling in cultured blastemal cells led to an upregulation of FGF20 expression. SMAD1 positively regulated the transcription of FGF20. Conclusion: BMP signaling positively regulates the transcription of FGF20 through SMAD1, thereby regulating tail regeneration in Gekko japonicus.
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基本信息:
DOI：10.16424/j.cnki.cn32-1807/r.2026.02.001
中图分类号:R-332
引用信息:
[1]赵彧,徐嫣然,王引,等.BMP信号上调FGF20促进多疣壁虎断尾再生[J].南通大学学报(医学版),2026,46(02):105-110.DOI:10.16424/j.cnki.cn32-1807/r.2026.02.001.
基金信息:
国家自然科学基金资助项目(32100380); 大学生创新创业训练计划项目(S202510304171)
2026-03-15
2026-03-15