南通大学学报(医学版)

目的 :建立皮层神经元缺氧损伤的模型 ,为抗缺氧研究提供可靠的实验方法。方法 :采用形态学观察和 MTT比色分析比较了不同密度、不同缺氧时间对培养神经元形态及活性的影响 ;同时观察了银杏内酯、人参皂甙预处理对神经元缺氧损伤的保护作用。结果 :( 1)在进行原代皮层神经元培养时 ,细胞接种的密度以 1.6× 10 6～ 2 .0× 10 6个细胞 /ml为好。( 2 )随着缺氧时间延长 ,神经元活性降低 ,细胞死亡率增加。 ( 3 )银杏内酯和人参皂甙预处理均能使缺氧 8h的神经元比单纯缺氧对照组活性明显升高。结论 :实验结果稳定 ,重复性好 ,是离体抗缺氧研究的一种较理想的实验方法

Aim: To establish a hypoxia model of cerebral neuron culture and supply a reliable method to the research of the prevention of hypoxia. Method: Morphological observation and MTT assay was used to compare the effect of different cell density and duration of hypoxia on the cultured neurons; Meanwhile,the prevention of the pretreatment with ginkgolide B and ginsenoside was also observed. Result: (1) It was proper in the density of 1.6×10 6².0×10 6 cell/ml of primary cultured cortical neurons. (2) The neuronal viability decreased or the cell death rate increased for longer exposure duration of hypoxia. (3) The neuronal viability could be enhanced significantly by the pretreatment with ginkgolides and ginsenosides for 8h. Conclusion:The assay described here is stable and can be repeated well. It is an ideal method to research hypoxia in vitro.