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目的 :探讨红景天甙对活性氧致心肌细胞损伤的保护作用及其机制。方法 :在应用低浓度过氧化氢处理原代培养乳鼠心肌细胞 ,建立心肌细胞氧化损伤模型的基础上 ,通过检测培养介质中乳酸脱氢酶 (LDH)活力和电镜观察细胞超微结构的方法评价红景天甙对心肌细胞的保护作用 ,并通过测定丙二醛 (MDA)含量和 Na+,K+-ATPase活力探讨其机制。结果 :过氧化氢对心肌细胞有显著的损伤作用。红景天甙可使培养介质中的 L DH和 MDA水平显著下降 ,细胞 Na+,K+-ATPase活力显著提高 ,同时细胞超微结构得到显著改善。结论 :红景天甙对过氧化氢致心肌细胞损伤有保护作用 ,机理与其抑制脂质过氧化和提高钠泵活力的作用有关。
Abstract：Objective:To determine whether the administration of salidroside is able to protect cardiomyocytes agonist oxidative stress. Methods:An experimental model of oxidative injury was established by using cultured neonatal rat cardiomyocytes.Myocytes damages were estimated by lactate dehydrogenase (LDH) release and ultrastructural morphological alteration. Malondialdehyde (MDA) content was measured as an index of lipid perioxidation. The activity of Na +,K +-ATPase was also assayed.Resultes:Salidroside treatment significantly decreased LDH leakage and MDA production and restored activities of Na +,K +-ATPase to control level.Conclusion:Pretreatment with salidroside protects cardiomyocytes from H 2O 2 induced injury, and the beneficial effect of salidroside may be related to its antioxidant properties and thus protect Na +,K +-ATPase from oxidative stress.
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基本信息:
中图分类号:R285
引用信息:
[1]邹琛,吴翔,姜敏辉,王政华,宗刚军.红景天甙对乳鼠心肌细胞过氧化损伤的保护作用[J].南通医学院学报,2003(04):391-393.